Click a colour below to change the separator colour.
Once you've chosen, drag the icon to the bookmarks bar and you're done!
This is a transparent spacer, no colour to select for this!
Just drag the blank box on the left to your bookmarks for a transparent spacer
Use the sidebar on the left to choose an icon for your Google Chrome bookmark separator
Decide which colour you want your separator to be and click on it to change the preview
Drag your chosen icon to the bookmarks bar - use as many icons/colours as you like